Optimizing DNA transfection requires balancing multiple variables such as cell density, reagent concentration, and DNA amount – often through time-consuming iterations.
Using the CELLCYTE X live-cell imaging platform, transfection efficiency was monitored in real time through expression of the fluorescent reporter mCherry in HEK 293T/17 cells. The system enabled precise evaluation of both penetrance (proportion of cells transfected) and expressivity (level of transgene expression per cell), streamlining the development of an optimized transfection protocol.
Learn more about how:
CELLCYTE X accelerates optimization of transfection workflows
Real-time imaging tracks reporter expression dynamics
Automated analysis quantifies both penetrance and expressivity
